Regulatory

Part:BBa_K944002:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)

Transcriptional Regulator for Cyanide Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 821
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 1730
    Illegal AgeI site found at 1686
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 511


Design Notes

This part had 3 illegal Pst1 restriction sites.


Source

This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies.

References

Luque-Almagro V. M., Huertas M. J., Sáez L. P., Luque-Romero M. M., Moreno-Vivián C., Castillo F., Roldán M. D., Blasco R. (2008). Characterization of the Pseudomonas pseudoalcaligenes CECT5344 cyanase, an enzyme that is not essential for cyanide assimilation. Appl. Environ. Microbiol. 74, 6280–6288. doi: 10.1128/AEM.00916-08.